BPC-157 (Body Protection Compound-157) is a synthetic 15-amino acid peptide that comes as a lyophilized (freeze-dried) powder in vials typically ranging from 2mg to 10mg. Before you can inject it, you need to reconstitute it with bacteriostatic water (BAC water). This guide walks through every step.
What You Need
Before reconstituting BPC-157, gather the following supplies:
Bacteriostatic water (BAC water): This is sterile water preserved with 0.9% benzyl alcohol, which inhibits bacterial growth and extends the shelf life of your reconstituted peptide. Do NOT use regular sterile water — it has no preservative and will contaminate.
Insulin syringes: U-100 syringes (100 units per mL) are standard. Use a fresh syringe for each draw.
Alcohol swabs: Wipe the rubber stopper of both your BAC water vial and your peptide vial before inserting any needle.
The peptide vial: BPC-157 arrives as a white powder. Most vials are 5mg or 10mg.
The Reconstitution Formula
The key calculation: how many mL of BAC water to add to achieve your target concentration.
Standard formula:
```
Concentration (mg/mL) = Vial size (mg) ÷ BAC water volume (mL)
Dose volume (mL) = Desired dose (mg) ÷ Concentration (mg/mL)
```
Common example — 5mg BPC-157 vial:
- Add 1mL BAC water → concentration = 5mg/mL = 5000mcg/mL
- For a 250mcg dose: 250 ÷ 5000 = 0.05mL = 5 units on a U-100 syringe
- For a 500mcg dose: 500 ÷ 5000 = 0.10mL = 10 units on a U-100 syringe
Adding more BAC water reduces concentration (making doses easier to measure), while adding less increases concentration. For BPC-157, most researchers add 1-2mL.
Step-by-Step Reconstitution
Step 1: Wipe the rubber stopper of your BAC water vial with an alcohol swab. Let it dry for 30 seconds.
Step 2: Draw the desired amount of BAC water into a clean insulin syringe. For a 5mg vial, draw 1mL (100 units on a U-100 syringe).
Step 3: Wipe the rubber stopper of your BPC-157 vial with a fresh alcohol swab.
Step 4: Insert the needle at an angle and inject the BAC water slowly down the side of the vial. Do not spray directly onto the powder — this can degrade the peptide.
Step 5: Gently swirl (do NOT shake) until the powder fully dissolves. The solution should be clear and colorless.
Step 6: Label the vial with the date and concentration. Store in the refrigerator at 2-8°C.
Dosage Reference for BPC-157
Standard research dosage: 250mcg–500mcg per injection, once or twice daily.
Weight-based dosing (sometimes used in research protocols): 2.5–10mcg/kg body weight.
For a 80kg (176lb) person at 5mcg/kg: 80 × 5 = 400mcg per dose.
Injection sites: BPC-157 is most commonly injected subcutaneously (SubQ) — pinch the skin around the belly or thigh and inject at a 45° angle. Some researchers inject near the injury site, though systemic effects are well-documented even with distant injection.
Cycle length: Most research protocols run 4-12 weeks. BPC-157 does not suppress natural hormones and does not require PCT.
Storage and Shelf Life
Lyophilized (before reconstitution): Store at -20°C (freezer) for long-term storage, or at 4°C (refrigerator) for up to 24 months. Keep away from light.
Reconstituted: Store at 4°C (refrigerator). Shelf life: approximately 4-6 weeks when stored in BAC water. Do not freeze reconstituted peptide more than once — freeze-thaw cycles degrade it.
Signs of degradation: Color change (yellow/brown tint), visible particles, or cloudiness after mixing typically indicate contamination or degradation. Discard and do not use.
Key Takeaways
Reconstituting BPC-157 is straightforward once you understand the math. The key points: use bacteriostatic water (not regular sterile water), inject BAC water gently down the side of the vial, never shake, and store reconstituted peptide in the refrigerator. Use the PeptideWiki calculator above to automatically calculate your draw volume for any vial size and dose.